s1nucleasemapping

由 MR Green 著作 · 2021 · 被引用 4 次 — This protocol provides details for nuclease S1 mapping of mRNA using a uniformly labeled, single-stranded DNA probe. DNA-RNA hybrids are ...

由 BR Mini-Protean 著作 · 2005 — The method can be used to quantify RNAs, to map the positions of introns and to identify the locations of 5′ and 3′ ends of mRNAs on cloned DNA templates.

The first step in mapping the transcriptional start site by S1 nuclease treatment is to clone the upstream region of the gene into an M13 vector. Next, single- ...

2011年8月19日 — S-1 mapping of an intron site. The nuclease digests the unhybridized intronic DNA, which is not represented in the RNA. The size of the labeled ...

由 S Viville 著作 · 1996 — S1 is used to recognize and cut mismatches or unannealed regions and the products are analyzed on a denaturing acrylamide gel. A number of different uses of the ...

由 TC King 著作 · 1983 · 被引用 81 次 — S 1 nuclease mapping was used to assess rRNA proc- essing in Escherichia coli. Single-stranded DNA probes complementary to he sequences ...

2023年1月21日 — 3. • Nuclease-S1 mapping is used to map the locations of the ends of RNA molecules and of any splice points within them in relation to specific ...

... S1 nuclease assays, are an extremely sensitive method for the detection, quantitation and mapping of specific RNAs in a complex mixture of total cellular RNA.

When the probe is a DNA molecule, S1 nuclease is used; when the probe is RNA ... Nuclease protection assays are used to map 內含子s and 5' and 3' ends of ...